Chapter title |
TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE
|
---|---|
Chapter number | 13 |
Book title |
Protein Gel Detection and Imaging
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8745-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8744-3, 978-1-4939-8745-0
|
Authors |
Na Na, Jin Ouyang, Na, Na, Ouyang, Jin |
Abstract |
Polyacrylamide gel electrophoresis (PAGE) has become one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. For simple, convenient, and ultrasensitive detection of proteins after PAGE, a novel enhanced photoluminescent (PL) imaging method was developed. Thioglycolic acid (TGA)-capped CdTe quantum dots (QDs) and the enhancer reagent tetramethylethylenediamine (TEMED) were introduced, achieving the direct detection of various proteins in native 1-DE, 2-DE and SDS-PAGE. Here we describe the general protocol of TEMED enhanced PL imaging by QDs, including materials, practical procedures, and some notes. |
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