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Chapter title |
A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins
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Chapter number | 18 |
Book title |
Protein Gel Detection and Imaging
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Published in |
Methods in molecular biology, August 2018
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DOI | 10.1007/978-1-4939-8745-0_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8744-3, 978-1-4939-8745-0
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Authors |
Jelena Bogdanović Pristov, Ivan Spasojević, Bogdanović Pristov, Jelena, Spasojević, Ivan |
Abstract |
Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)-PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 1 | 50% |
Unknown | 1 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Unknown | 1 | 50% |