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Chapter title |
Detection of Phosphoproteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain
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Chapter number | 9 |
Book title |
Protein Gel Detection and Imaging
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Published in |
Methods in molecular biology, August 2018
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DOI | 10.1007/978-1-4939-8745-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8744-3, 978-1-4939-8745-0
|
Authors |
Sun-Young Hwang, Xu Wang, Jung-Kap Choi, Hwang, Sun-Young, Wang, Xu, Choi, Jung-Kap |
Abstract |
In order to detect phosphoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), an easy and fast fluorescent detection method is described. 8-Quinolinol can form ternary complexes in the gel matrix contributed by the affinity of aluminum ion to the phosphate groups on the proteins and the metal chelating property of 8-Quinolinol, exhibiting strong fluorescence in ultraviolet light. It can visualize as little as 4-8 ng of α-casein and β-casein, 15-31 ng of ovalbumin and κ-casein within 70 min. The approach utilizing 8-quinolinol could be an alternative staining method for phosphoproteomics. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 1 | 100% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 100% |