Chapter title |
A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue
|
---|---|
Chapter number | 5 |
Book title |
Protein Gel Detection and Imaging
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8745-0_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8744-3, 978-1-4939-8745-0
|
Authors |
Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, Tian-yun Wang, Dong, Wei-hua, Wang, Fang, Zhang, Jun-he, Zhou, Yan-sheng, Zhang, Ling-ye, Wang, Tian-yun |
Abstract |
Most traditional post-electrophoretic processes need several hours to several days to finish the whole staining process and traditional staining solutions all contain methanol, acetic acid, or phosphoric acid, which not only produce the unpleasant smell but also cause environmental pollution. Here a fixation-free, fast protein staining method in sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol includes only staining and quick washing steps, can be completed in 0.5 h. It has a sensitivity of 10 ng. In addition, the dye stain does not contain any acid or methanol. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 20% |
Student > Bachelor | 2 | 20% |
Student > Master | 2 | 20% |
Student > Doctoral Student | 1 | 10% |
Professor | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |
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Unknown | 2 | 20% |