Chapter title |
Transient Expression Systems in Plants: Potentialities and Constraints.
|
---|---|
Chapter number | 18 |
Book title |
Advanced Technologies for Protein Complex Production and Characterization
|
Published in |
Advances in experimental medicine and biology, May 2016
|
DOI | 10.1007/978-3-319-27216-0_18 |
Pubmed ID | |
Book ISBNs |
978-3-31-927214-6, 978-3-31-927216-0
|
Authors |
Tomas Canto, Canto, Tomas |
Editors |
M. Cristina Vega |
Abstract |
Plants have been used from old to extract and isolate by different means the products of interest that they store. In recent years new techniques have emerged that allow the use of plants as factories to overexpress transiently and often efficiently, specific genes of interest, either endogenous or foreign, in their native form or modified. These techniques allow and facilitate the targeted purification of gene products for research and commercial purposes without resorting to lengthy, time-consuming and sometimes challenging plant stable transformations, while avoiding some of their associated regulatory constraints. In this chapter we describe the main strategies available for the transient expression of gene sequences and their encoded products in plants. We discuss biological issues affecting transient expression, including resistance responses elicited by the plant against sequences that it recognizes naturally as foreign, and ways to neutralize them. We also discuss the relative advantages of each expression strategy as well as their inherent drawbacks and technical limitations, and how to partially prevent or overcome them, whenever possible. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 49 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 9 | 18% |
Student > Ph. D. Student | 5 | 10% |
Researcher | 5 | 10% |
Student > Master | 4 | 8% |
Student > Doctoral Student | 1 | 2% |
Other | 1 | 2% |
Unknown | 24 | 49% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 24% |
Agricultural and Biological Sciences | 7 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 8% |
Chemical Engineering | 1 | 2% |
Social Sciences | 1 | 2% |
Other | 0 | 0% |
Unknown | 24 | 49% |