Chapter title |
Oxygen Transport to Tissue XXXVIII
|
---|---|
Chapter number | 32 |
Book title |
Oxygen Transport to Tissue XXXVIII
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-38810-6_32 |
Pubmed ID | |
Book ISBNs |
978-3-31-938808-3, 978-3-31-938810-6
|
Authors |
Bragin, D E, Peng, Z, Bragina, O A, Statom, G L, Kameneva, M V, Nemoto, E M, D. E. Bragin, Z. Peng, O. A. Bragina, G. L. Statom, M. V. Kameneva, E. M. Nemoto |
Editors |
Qingming Luo, Lin Z. Li, David K. Harrison, Hua Shi, Duane F. Bruley |
Abstract |
Nanomolar intravascular concentrations of drag-reducing polymers (DRP) have been shown to improve hemodynamics and survival in animal models of ischemic myocardium and limb, but the effects of DRP on the cerebral microcirculation have not yet been studied. We recently demonstrated that DRP enhance microvascular flow in normal rat brain and hypothesized that it would restore impaired microvascular perfusion and improve outcomes after focal ischemia and traumatic brain injury (TBI). We studied the effects of DRP (high molecular weight polyethylene oxide, 4000 kDa, i.v. at 2 μg/mL of blood) on microcirculation of the rat brain: (1) after permanent middle cerebral artery occlusion (pMCAO); and (2) after TBI induced by fluid percussion. Using in vivo two-photon laser scanning microscopy (2PLSM) over the parietal cortex of anesthetized rats we showed that both pMCAO and TBI resulted in progressive decrease in microvascular circulation, leading to tissue hypoxia (NADH increase) and increased blood brain barrier (BBB) degradation. DRP, injected post insult, increased blood volume flow in arterioles and red blood cell (RBC) flow velocity in capillaries mitigating capillary stasis, tissue hypoxia and BBB degradation, which improved neuronal survival (Fluoro-Jade B, 24 h) and neurologic outcome (Rotarod, 1 week). Improved microvascular perfusion by DRP may be effective in the treatment of ischemic stroke and TBI. |
X Demographics
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 31 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 16% |
Student > Ph. D. Student | 4 | 13% |
Professor > Associate Professor | 2 | 6% |
Student > Master | 2 | 6% |
Student > Doctoral Student | 1 | 3% |
Other | 4 | 13% |
Unknown | 13 | 42% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 9 | 29% |
Neuroscience | 7 | 23% |
Unspecified | 1 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Unknown | 13 | 42% |