Chapter title |
Skeletal Muscle Oxygenation Measured by EPR Oximetry Using a Highly Sensitive Polymer-Encapsulated Paramagnetic Sensor.
|
---|---|
Chapter number | 46 |
Book title |
Oxygen Transport to Tissue XXXVIII
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-38810-6_46 |
Pubmed ID | |
Book ISBNs |
978-3-31-938808-3, 978-3-31-938810-6
|
Authors |
H. Hou, N. Khan, M. Nagane, S. Gohain, E. Y. Chen, L. A. Jarvis, P. E. Schaner, B. B. Williams, A. B. Flood, H. M. Swartz, P. Kuppusamy |
Editors |
Qingming Luo, Lin Z. Li, David K. Harrison, Hua Shi, Duane F. Bruley |
Abstract |
We have incorporated LiNc-BuO, an oxygen-sensing paramagnetic material, in polydimethylsiloxane (PDMS), which is an oxygen-permeable, biocompatible, and stable polymer. We fabricated implantable and retrievable oxygen-sensing chips (40 % LiNc-BuO in PDMS) using a 20-G Teflon tubing to mold the chips into variable shapes and sizes for in vivo studies in rats. In vitro EPR measurements were used to test the chip's oxygen response. Oxygen induced linear and reproducible line broadening with increasing partial pressure (pO2). The oxygen response was similar to that of bare (unencapsulated) crystals and did not change significantly on sterilization by autoclaving. The chips were implanted in rat femoris muscle and EPR oximetry was performed repeatedly (weekly) for 12 weeks post-implantation. The measurements showed good reliability and reproducibility over the period of testing. These results demonstrated that the new formulation of OxyChip with 40 % LiNc-BuO will enable the applicability of EPR oximetry for long-term measurement of oxygen concentration in tissues and has the potential for clinical applications. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 25% |
Student > Ph. D. Student | 2 | 13% |
Student > Doctoral Student | 1 | 6% |
Student > Bachelor | 1 | 6% |
Professor | 1 | 6% |
Other | 2 | 13% |
Unknown | 5 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Agricultural and Biological Sciences | 2 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Chemistry | 1 | 6% |
Other | 2 | 13% |
Unknown | 7 | 44% |