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Mendeley readers
Chapter title |
Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis.
|
---|---|
Chapter number | 11 |
Book title |
In Vitro Mutagenesis
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6472-7_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6470-3, 978-1-4939-6472-7
|
Authors |
Charlotte E. Key, Derek J. Fisher |
Editors |
Andrew Reeves |
Abstract |
Dissecting the contribution of genes to virulence in fulfillment of Molecular Koch's postulates is essential for developing prevention and treatment strategies for bacterial pathogens. This chapter will discuss the application of a targeted, intron-based insertional mutagenesis method for creating mutants in the obligate, intracellular bacterial pathogen Chlamydia trachomatis. The methods employed for intron targeting, mutant selection, and mutant verification will be outlined including available selection markers, gene targeting strategies, and potential pitfalls. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 21% |
Student > Doctoral Student | 2 | 14% |
Student > Bachelor | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Professor | 1 | 7% |
Other | 2 | 14% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 29% |
Immunology and Microbiology | 3 | 21% |
Medicine and Dentistry | 2 | 14% |
Psychology | 1 | 7% |
Nursing and Health Professions | 1 | 7% |
Other | 0 | 0% |
Unknown | 3 | 21% |