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In Vitro Mutagenesis

Overview of attention for book
Cover of 'In Vitro Mutagenesis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.
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    Chapter 2 Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9.
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    Chapter 3 Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.
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    Chapter 4 All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.
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    Chapter 5 CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium.
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    Chapter 6 Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.
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    Chapter 7 Generation of Stable Knockout Mammalian Cells by TALEN-Mediated Locus-Specific Gene Editing.
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    Chapter 8 Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-cloned Technology.
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    Chapter 9 Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes.
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    Chapter 10 Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells.
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    Chapter 11 Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis.
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    Chapter 12 In Silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein-Cofactor and Protein-Protein Functional Relationships.
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    Chapter 13 In Silico Prediction of Deleteriousness for Nonsynonymous and Splice-Altering Single Nucleotide Variants in the Human Genome.
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    Chapter 14 In Silico Methods for Analyzing Mutagenesis Targets.
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    Chapter 15 Methods for Detecting Critical Residues in Proteins.
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    Chapter 16 A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants.
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    Chapter 17 Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.
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    Chapter 18 Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors.
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    Chapter 19 PERMutation Using Transposase Engineering (PERMUTE): A Simple Approach for Constructing Circularly Permuted Protein Libraries.
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    Chapter 20 Transposon Insertion Mutagenesis for Archaeal Gene Discovery.
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    Chapter 21 Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis.
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    Chapter 22 Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.
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    Chapter 23 Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis.
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    Chapter 24 Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.
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    Chapter 25 Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
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    Chapter 26 An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.
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    Chapter 27 Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function.
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    Chapter 28 Step-By-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL.
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    Chapter 29 Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein-Cofactor and Protein-Protein Functional Relationships.
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    Chapter 30 Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions.
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    Chapter 31 Use of Random and Site-Directed Mutagenesis to Probe Protein Structure-Function Relationships: Applied Techniques in the Study of Helicobacter pylori.
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    Chapter 32 Novel Random Mutagenesis Method for Directed Evolution.
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    Chapter 33 Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent.
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    Chapter 34 Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR).
Attention for Chapter 21: Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis.
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Chapter title
Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis.
Chapter number 21
Book title
In Vitro Mutagenesis
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6472-7_21
Pubmed ID
27709585
Book ISBNs
978-1-4939-6470-3, 978-1-4939-6472-7
Authors

Gaurav Majumdar, Rendani Mbau, Vinayak Singh, Digby F. Warner, Marte Singsås Dragset, Raju Mukherjee

Editors

Andrew Reeves

Abstract

TnSeq, or transposon (Tn) insertion sequencing, is a powerful method for identifying the essential-as well as conditionally essential-regions in a genome, both coding and noncoding. The advent of accessible massively parallel DNA sequencing technologies in particular has resulted in the increased use of TnSeq-based approaches to elucidate various aspects of bacterial physiology and metabolism. Moreover, the availability of detailed protocols has enabled even nonspecialist laboratories to adapt and develop TnSeq approaches to address specific research questions. In this chapter, we describe a recently modified experimental protocol used in our laboratory for TnSeq in the major human pathogen, Mycobacterium tuberculosis, as well as the related non-pathogenic mycobacterium, M. smegmatis. The method, which was developed in close consultation with pioneers in the field of mycobacterial genetics, includes the steps involved in preparing a phage stock, generating a mutant library, selection of the library under a specific experimental condition, isolation of genomic DNA from the pooled population of mutants, amplification of the sites of Tn insertion and, finally, determining the essential genomic regions by next-generation sequencing.

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X Demographics

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 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 19%
Student > Master 7 16%
Student > Bachelor 4 9%
Student > Ph. D. Student 3 7%
Professor > Associate Professor 3 7%
Other 7 16%
Unknown 11 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 28%
Biochemistry, Genetics and Molecular Biology 9 21%
Immunology and Microbiology 5 12%
Arts and Humanities 1 2%
Social Sciences 1 2%
Other 2 5%
Unknown 13 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2018.
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#20,344,065
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Outputs from Methods in molecular biology
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