Chapter title |
Transposon Insertion Mutagenesis for Archaeal Gene Discovery.
|
---|---|
Chapter number | 20 |
Book title |
In Vitro Mutagenesis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6472-7_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6470-3, 978-1-4939-6472-7
|
Authors |
Saija Kiljunen, Maria I. Pajunen, Harri Savilahti, Kiljunen, Saija, Pajunen, Maria I., Savilahti, Harri |
Editors |
Andrew Reeves |
Abstract |
Archaea constitute the third domain of life, but studies on their physiology and other features have lagged behind bacteria and eukarya, largely due to the challenging biology of archaea and concomitant difficulties in methods development. The use of genome-wide en masse insertion mutagenesis is one of the most efficient means to discover the genes behind various biological functions, and such a methodology is described in this chapter for a model archaeon Haloferax volcanii. The strategy successfully employs efficient in vitro transposition in combination with gene targeting in vivo via homologous recombination. The methodology is general and should be transferable to other archaeal species. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 30% |
Student > Postgraduate | 2 | 20% |
Student > Bachelor | 1 | 10% |
Librarian | 1 | 10% |
Researcher | 1 | 10% |
Other | 1 | 10% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 40% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Arts and Humanities | 1 | 10% |
Chemical Engineering | 1 | 10% |
Unknown | 2 | 20% |