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Mendeley readers
| Chapter title |
Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).
|
|---|---|
| Chapter number | 8 |
| Book title |
Synthetic DNA
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6343-0_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6341-6, 978-1-4939-6343-0
|
| Authors |
Sunil Chandran |
| Editors |
Randall A. Hughes |
| Abstract |
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the ligase chain reaction (LCR). The LCR method utilizes non-overlapping DNA parts that are ligated together with the guidance of bridging oligos. Using this method, we have successfully assembled up to 20 DNA parts in a single reaction or DNA constructs up to 26 kb in size. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| China | 1 | 3% |
| Unknown | 31 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 21% |
| Student > Master | 5 | 15% |
| Student > Ph. D. Student | 4 | 12% |
| Student > Bachelor | 3 | 9% |
| Student > Postgraduate | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 9 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 48% |
| Agricultural and Biological Sciences | 5 | 15% |
| Chemistry | 2 | 6% |
| Engineering | 1 | 3% |
| Unknown | 9 | 27% |