While a fantastic tool, FML preparation is limited to high biomass/plentiful microbiomes. It is DNA greedy because it usually uses physical sonication to fragment DNA and it uses that DNA inefficiently because it relies on blunt ligation cloning (eg https:
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Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds https://t.co/YUptgU5rv6
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Once again! 😂🙌🏻
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RT @MilnerCentre: Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacter…