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Xylem

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Cover of 'Xylem'

Table of Contents

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    Book Overview
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    Chapter 1 Experimental and Theoretical Methods to Approach the Study of Vascular Patterning in the Plant Shoot.
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    Chapter 2 Strigolactone-mediated Stimulation of Secondary Xylem Proliferation in Stems.
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    Chapter 3 Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.
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    Chapter 4 Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis.
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    Chapter 5 Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
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    Chapter 6 VND6-induced Xylem Cell Differentiation in Arabidopsis Cell Cultures.
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    Chapter 7 Live Imaging of Developing Xylem In Planta.
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    Chapter 8 Immunolocalization in Secondary Xylem of Populus.
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    Chapter 9 Xylem
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    Chapter 10 Vascular Morphodynamics During Secondary Growth.
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    Chapter 11 Xylem Characterization Using Improved Pseudo-Schiff Propidium Iodide Staining of Whole Mount Samples and Confocal Laser-Scanning Microscopy.
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    Chapter 12 Chemical Imaging of Xylem by Raman Microspectroscopy.
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    Chapter 13 Using CellProfiler to Analyze and Quantify Vascular Morphology.
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    Chapter 14 Lignin Analysis by HPLC and FTIR.
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    Chapter 15 Carbohydrate Composition Analysis in Xylem.
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    Chapter 16 Structural Analysis of Cell Wall Polysaccharides Using PACE.
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    Chapter 17 Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy.
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    Chapter 18 Topochemical Analysis of Cell Wall Components by TOF-SIMS.
Attention for Chapter 5: Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
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Chapter title
Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
Chapter number 5
Book title
Xylem
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6722-3_5
Pubmed ID
Book ISBNs
978-1-4939-6720-9, 978-1-4939-6722-3
Authors

Masato Saito, Alif Meem Nurani, Yuki Kondo, Hiroo Fukuda

Editors

Miguel de Lucas, J. Peter Etchhells

Abstract

Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Other 1 25%
Student > Master 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 75%
Unknown 1 25%