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Xylem

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Cover of 'Xylem'

Table of Contents

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    Book Overview
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    Chapter 1 Experimental and Theoretical Methods to Approach the Study of Vascular Patterning in the Plant Shoot.
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    Chapter 2 Strigolactone-mediated Stimulation of Secondary Xylem Proliferation in Stems.
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    Chapter 3 Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.
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    Chapter 4 Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis.
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    Chapter 5 Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
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    Chapter 6 VND6-induced Xylem Cell Differentiation in Arabidopsis Cell Cultures.
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    Chapter 7 Live Imaging of Developing Xylem In Planta.
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    Chapter 8 Immunolocalization in Secondary Xylem of Populus.
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    Chapter 9 Xylem
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    Chapter 10 Vascular Morphodynamics During Secondary Growth.
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    Chapter 11 Xylem Characterization Using Improved Pseudo-Schiff Propidium Iodide Staining of Whole Mount Samples and Confocal Laser-Scanning Microscopy.
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    Chapter 12 Chemical Imaging of Xylem by Raman Microspectroscopy.
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    Chapter 13 Using CellProfiler to Analyze and Quantify Vascular Morphology.
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    Chapter 14 Lignin Analysis by HPLC and FTIR.
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    Chapter 15 Carbohydrate Composition Analysis in Xylem.
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    Chapter 16 Structural Analysis of Cell Wall Polysaccharides Using PACE.
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    Chapter 17 Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy.
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    Chapter 18 Topochemical Analysis of Cell Wall Components by TOF-SIMS.
Attention for Chapter 8: Immunolocalization in Secondary Xylem of Populus.
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Chapter title
Immunolocalization in Secondary Xylem of Populus.
Chapter number 8
Book title
Xylem
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6722-3_8
Pubmed ID
Book ISBNs
978-1-4939-6720-9, 978-1-4939-6722-3
Authors

Suzanne Gerttula, Andrew Groover

Editors

Miguel de Lucas, J. Peter Etchhells

Abstract

Immunolocalization can be used to precisely visualize the location of specific proteins, cell wall components, or any other molecules within cells or tissues for which specific antibodies are available. Here we describe an immunolocalization protocol for tissue sections of woody Populus stems. The protocol includes descriptions of the required sectioning, fixation, probing, detection, and imaging parameters, as well suggested controls useful in interpreting results.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Student > Bachelor 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 100%