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Mendeley readers
| Chapter title |
Xylem Characterization Using Improved Pseudo-Schiff Propidium Iodide Staining of Whole Mount Samples and Confocal Laser-Scanning Microscopy.
|
|---|---|
| Chapter number | 11 |
| Book title |
Xylem
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6722-3_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6720-9, 978-1-4939-6722-3
|
| Authors |
Mario Coiro, Elisabeth Truernit |
| Editors |
Miguel de Lucas, J. Peter Etchhells |
| Abstract |
An improved pseudo-Schiff propidium iodide staining technique well suited for, but not limited to, the visualization of xylem cell walls in whole mount samples is presented. The pseudo-Schiff reaction results in covalent binding of the fluorescent dye propidium iodide to cell walls. This stable linkage permits the use of clearing agents after staining, which is itself improved following pretreatment of the plant tissue. A subsequent acid alcohol washing step eliminates unbound propidium iodide to reduce background fluorescence. The method can be used for characterizing xylem cell structure in different organs and species without the need for tissue sectioning. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 57% |
| Unknown | 3 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Arts and Humanities | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Nursing and Health Professions | 1 | 14% |
| Agricultural and Biological Sciences | 1 | 14% |
| Unknown | 3 | 43% |