| Chapter title |
Vascular Morphodynamics During Secondary Growth.
|
|---|---|
| Chapter number | 10 |
| Book title |
Xylem
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6722-3_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6720-9, 978-1-4939-6722-3
|
| Authors |
Pierre Barbier de Reuille, Laura Ragni |
| Editors |
Miguel de Lucas, J. Peter Etchhells |
| Abstract |
Quantification of vascular morphodynamics during secondary growth has been hampered by the scale of the process. Even in the tiny model plant Arabidopsis thaliana, the xylem can include more than 2000 cells in a single cross section, rendering manual counting impractical. Moreover, due to its deep location, xylem is an inaccessible tissue, limiting live imaging. A novel method to visualize and measure secondary growth progression has been proposed: "the Quantitative Histology" approach. This method is based on a detailed anatomical atlas, and image segmentation coupled with machine learning to automatically extract cell shapes and identify cell type. Here we present a new version of this approach, with a user-friendly interface implemented in the open source software LithoGraphX. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 25% |
| Student > Bachelor | 3 | 25% |
| Student > Ph. D. Student | 2 | 17% |
| Unspecified | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 50% |
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Unspecified | 1 | 8% |
| Unknown | 2 | 17% |