Chapter title |
Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications
|
---|---|
Chapter number | 4 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, May 2016
|
DOI | 10.1007/978-1-4939-3618-2_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Rajpal S. Kashyap, Shradha S. Bhullar, Nitin H. Chandak, Girdhar M. Taori |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Synthetic peptide-based diagnosis of Chikungunya can be an efficient and more accessible approach in immunodiagnostics. Here, we describe the identification of Chikungunya-specific 40 kD protein for development of synthetic peptide-based enzyme-linked immunosorbent assay for the detection of Chikungunya virus-specific antibodies in the patient's sample. The total sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profile of the patient's sample can be done to identify specific protein bands. The identified proteins can be subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for characterization. After characterization, immunogenic peptides can be designed using softwares and subsequently synthesized chemically. The peptides can be used to develop more specific, sensitive, and simpler diagnostic assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 6% |
Unknown | 16 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 24% |
Other | 3 | 18% |
Student > Master | 2 | 12% |
Student > Doctoral Student | 2 | 12% |
Professor > Associate Professor | 1 | 6% |
Other | 1 | 6% |
Unknown | 4 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 24% |
Medicine and Dentistry | 3 | 18% |
Agricultural and Biological Sciences | 2 | 12% |
Arts and Humanities | 1 | 6% |
Social Sciences | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 29% |