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Chikungunya Virus

Overview of attention for book
Cover of 'Chikungunya Virus'

Table of Contents

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    Book Overview
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    Chapter 1 Evolution and Epidemiology of Chikungunya Virus
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    Chapter 2 Molecular Epidemiology of Chikungunya Virus by Sequencing
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    Chapter 3 Chikungunya Virus
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    Chapter 4 Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications
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    Chapter 5 Expression and Purification of E2 Glycoprotein from Insect Cells (Sf9) for Use in Serology
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    Chapter 6 Diagnostic Methods for CHIKV Based on Serological Tools
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    Chapter 7 Utilization and Assessment of Throat Swab and Urine Specimens for Diagnosis of Chikungunya Virus Infection
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    Chapter 8 Propagation of Chikungunya Virus Using Mosquito Cells
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    Chapter 9 Infectious Viral Quantification of Chikungunya Virus—Virus Plaque Assay
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    Chapter 10 Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay
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    Chapter 11 Chikungunya Virus
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    Chapter 12 Chikungunya Virus
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    Chapter 13 Chikungunya Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence Assay
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    Chapter 14 Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
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    Chapter 15 Chikungunya Virus
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    Chapter 16 Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection: Preparation of Peptides for Analysis
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    Chapter 17 Bioinformatics Based Approaches to Study Virus–Host Interactions During Chikungunya Virus Infection
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    Chapter 18 T-Cell Epitope Prediction of Chikungunya Virus
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    Chapter 19 Mouse Models of Chikungunya Virus
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    Chapter 20 Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit
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    Chapter 21 Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues
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    Chapter 22 Antiviral Strategies Against Chikungunya Virus
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    Chapter 23 A Real-Time Cell Analyzing Assay for Identification of Novel Antiviral Compounds against Chikungunya Virus
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    Chapter 24 Using Bicistronic Baculovirus Expression Vector System to Screen the Compounds That Interfere with the Infection of Chikungunya Virus
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    Chapter 25 Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
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    Chapter 26 Reverse Genetics Approaches for Chikungunya Virus
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    Chapter 27 Chikungunya Virus
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    Chapter 28 Chikungunya Virus
Attention for Chapter 14: Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
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Chapter title
Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
Chapter number 14
Book title
Chikungunya Virus
Published in
Methods in molecular biology, May 2016
DOI 10.1007/978-1-4939-3618-2_14
Pubmed ID
Book ISBNs
978-1-4939-3616-8, 978-1-4939-3618-2
Authors

Chang-Kweng Lim

Editors

Justin Jang Hann Chu, Swee Kim Ang

Abstract

Virus isolation and purification is an invaluable technique in virology to detect and characterize viruses. This chapter describes a large-scale Chikungunya virus (CHIKV) propagation and purification methods by using discontinuous sucrose gradient, and sample preparation for transmission electron microscopy. Sucrose-banding yields large quantities of high-titer (10(10) pfu/ml) CHIKV stocks. Such stocks are stable for years when stored at -70 °C.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 22%
Student > Doctoral Student 3 13%
Student > Ph. D. Student 3 13%
Other 2 9%
Professor > Associate Professor 2 9%
Other 4 17%
Unknown 4 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 17%
Agricultural and Biological Sciences 4 17%
Immunology and Microbiology 3 13%
Medicine and Dentistry 2 9%
Nursing and Health Professions 1 4%
Other 3 13%
Unknown 6 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2023.
All research outputs
#21,625,765
of 24,137,435 outputs
Outputs from Methods in molecular biology
#10,424
of 13,615 outputs
Outputs of similar age
#299,778
of 343,904 outputs
Outputs of similar age from Methods in molecular biology
#15
of 17 outputs
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So far Altmetric has tracked 13,615 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.