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Mendeley readers
| Chapter title |
Reverse Genetics Approaches for Chikungunya Virus
|
|---|---|
| Chapter number | 26 |
| Book title |
Chikungunya Virus
|
| Published in |
Methods in molecular biology, May 2016
|
| DOI | 10.1007/978-1-4939-3618-2_26 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
| Authors |
Patchara Phuektes, Justin Jang Hann Chu |
| Editors |
Justin Jang Hann Chu, Swee Kim Ang |
| Abstract |
Reverse genetic systems based on an infectious cDNA clone, a double-stranded copy of the viral genome carried on a plasmid vector, have greatly enhanced the understanding of RNA virus biology by facilitating genetic manipulation of viral RNA genomes. To date, infectious cDNA clones of Chikungunya virus (CHIKV) have been constructed using different combinations of plasmid vectors and/or bacterial host strains. Here, we describe our approaches for the construction of infectious cDNA clones of CHIKV and the protocol for genetic manipulation of the clones by site-directed mutagenesis. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 8% |
| Unknown | 12 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 15% |
| Student > Doctoral Student | 2 | 15% |
| Student > Master | 2 | 15% |
| Researcher | 2 | 15% |
| Student > Postgraduate | 2 | 15% |
| Other | 1 | 8% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 23% |
| Immunology and Microbiology | 2 | 15% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Arts and Humanities | 1 | 8% |
| Economics, Econometrics and Finance | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 23% |