| Chapter title |
Diagnostic Methods for CHIKV Based on Serological Tools
|
|---|---|
| Chapter number | 6 |
| Book title |
Chikungunya Virus
|
| Published in |
Methods in molecular biology, May 2016
|
| DOI | 10.1007/978-1-4939-3618-2_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
| Authors |
Paolo Gaibani, Maria Poala Landini, Vittorio Sambri |
| Editors |
Justin Jang Hann Chu, Swee Kim Ang |
| Abstract |
This chapter presents the most commonly used serological methods for the diagnosis of Chikungunya virus (CHIKV) infection in humans. CHIKV is a mosquito-borne Alphavirus widely distributed in the tropical and subtropical regions of Africa, Asia, and America. CHIKV infection in human causes acute febrile illness frequently accompanied by severe joint pain. Most of the infected patients may develop chronic arthralgia that may persist for several months or years. Laboratory diagnosis of CHIKV infection is mainly based on molecular and serological tests. The serological tests represent a valuable tool for diagnosis and epidemiological studies. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA) are simple, rapid, and sensitive techniques widely used for the diagnosis of CHIKV infection. However, these methods represent a screening tool and often require confirmation by a second-line assays. Serum virus neutralization assay is more specific than ELISA and IFA tests and is considered a confirmatory test. Neutralization assay is employed to determine the titer of virus neutralizing antibodies against CHIKV in patients' sera. The basis of microneutralization assay (MNA), results interpretation, and procedures will be illustrated in this chapter. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Germany | 1 | 2% |
| Saint Kitts and Nevis | 1 | 2% |
| Unknown | 43 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 13% |
| Other | 5 | 11% |
| Student > Ph. D. Student | 4 | 9% |
| Student > Master | 4 | 9% |
| Student > Bachelor | 3 | 7% |
| Other | 8 | 17% |
| Unknown | 16 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 8 | 17% |
| Biochemistry, Genetics and Molecular Biology | 7 | 15% |
| Agricultural and Biological Sciences | 3 | 7% |
| Immunology and Microbiology | 3 | 7% |
| Nursing and Health Professions | 1 | 2% |
| Other | 5 | 11% |
| Unknown | 19 | 41% |