| Chapter title |
Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays
|
|---|---|
| Chapter number | 11 |
| Book title |
Small Molecule Microarrays
|
| Published in |
Methods in molecular biology, November 2016
|
| DOI | 10.1007/978-1-4939-6584-7_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6582-3, 978-1-4939-6584-7
|
| Authors |
Colleen M. Connelly, Fardokht A. Abulwerdi, John S. Schneekloth Jr., John S. SchneeklothJr., John S. Schneekloth |
| Editors |
Mahesh Uttamchandani, Shao Q. Yao |
| Abstract |
New methods to identify RNA-binding small molecules open yet unexplored opportunities for the pharmacological modulation of RNA-driven biology and disease states. One such approach is the use of small molecule microarrays (SMMs). Typically, SMMs are generated by spatially arraying and covalently linking a library of small molecules to a glass surface. Next, incubation of the arrays with a fluorescently labeled RNA reveals binding interactions that are detected upon slide imaging. The relative ease with which SMMs are manufactured enables the screening of multiple oligonucleotides in parallel against tens of thousands of small molecules, providing information about both binding and selectivity of identified RNA-small molecule interactions. This approach is useful for screening a broad variety of structurally and functionally diverse RNAs. Here, we present a general method for the preparation and use of SMMs to rapidly identify small molecules that selectively bind to an RNA of interest. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 42 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 24% |
| Unspecified | 5 | 12% |
| Student > Ph. D. Student | 5 | 12% |
| Professor | 2 | 5% |
| Student > Master | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 16 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 24% |
| Chemistry | 6 | 14% |
| Unspecified | 5 | 12% |
| Agricultural and Biological Sciences | 2 | 5% |
| Mathematics | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 16 | 38% |