Chapter title |
Array-on-Array Strategy For Activity-Based Enzyme Profiling
|
---|---|
Chapter number | 9 |
Book title |
Small Molecule Microarrays
|
Published in |
Methods in molecular biology, November 2016
|
DOI | 10.1007/978-1-4939-6584-7_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6582-3, 978-1-4939-6584-7
|
Authors |
Brendan Fu-Long Sieow, Mahesh Uttamchandani |
Editors |
Mahesh Uttamchandani, Shao Q. Yao |
Abstract |
We describe a novel array on array strategy intended to enhance the throughput of enzymatic activity screening using microarrays. This strategy consists of spotting a first array with large droplets of enzymes with varying concentrations and subsequently spotting a second array with small droplets of fluorogenic substrate on top of the enzyme array. By varying the array on array spotting patterns of different classes of enzyme (e.g., proteases, phosphatases, kinases) and their corresponding fluorogenic substrates, we have the unprecedented ability for testing enzymes and mixed samples in a multiplexed fashion within a single microarray slide. This new approach enables rapid enzyme characterization building upon a one enzyme on one slide droplet-based screening concept previously established. |
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.