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Small Molecule Microarrays

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Cover of 'Small Molecule Microarrays'

Table of Contents

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    Book Overview
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    Chapter 1 The Expanding World of Small Molecule Microarrays
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    Chapter 2 Novel Substrates for Microarrays
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    Chapter 3 Fabrication of Bio-function-Preserved Saccharide Microarray Chips with Cyanuric Chloride as a Rotatable Linker
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    Chapter 4 Fabrication of Carbohydrate Microarrays by Boronate Formation
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    Chapter 5 Clickable Polymeric Coating for Glycan Microarrays
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    Chapter 6 A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene
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    Chapter 7 Label-Free Sensing on Microarrays
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    Chapter 8 Optical Microscopy for Detecting Binding on Small Molecule Microarrays
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    Chapter 9 Array-on-Array Strategy For Activity-Based Enzyme Profiling
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    Chapter 10 Protein–Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays
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    Chapter 11 Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays
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    Chapter 12 Profiling Phosphopeptide-Binding Domain Recognition Specificity Using Peptide Microarrays
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    Chapter 13 Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-Based Chemiluminescence Microarrays
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    Chapter 14 Large-Scale Interaction Profiling of Protein Domains Through Proteomic Peptide-Phage Display Using Custom Peptidomes
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    Chapter 15 Synthetic Glycan Microarrays
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    Chapter 16 Screening Mammalian Cells on a Hydrogel: Functionalized Small Molecule Microarray
Attention for Chapter 9: Array-on-Array Strategy For Activity-Based Enzyme Profiling
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Chapter title
Array-on-Array Strategy For Activity-Based Enzyme Profiling
Chapter number 9
Book title
Small Molecule Microarrays
Published in
Methods in molecular biology, November 2016
DOI 10.1007/978-1-4939-6584-7_9
Pubmed ID
Book ISBNs
978-1-4939-6582-3, 978-1-4939-6584-7
Authors

Brendan Fu-Long Sieow, Mahesh Uttamchandani

Editors

Mahesh Uttamchandani, Shao Q. Yao

Abstract

We describe a novel array on array strategy intended to enhance the throughput of enzymatic activity screening using microarrays. This strategy consists of spotting a first array with large droplets of enzymes with varying concentrations and subsequently spotting a second array with small droplets of fluorogenic substrate on top of the enzyme array. By varying the array on array spotting patterns of different classes of enzyme (e.g., proteases, phosphatases, kinases) and their corresponding fluorogenic substrates, we have the unprecedented ability for testing enzymes and mixed samples in a multiplexed fashion within a single microarray slide. This new approach enables rapid enzyme characterization building upon a one enzyme on one slide droplet-based screening concept previously established.