Chapter title |
Fabrication of Carbohydrate Microarrays by Boronate Formation
|
---|---|
Chapter number | 4 |
Book title |
Small Molecule Microarrays
|
Published in |
Methods in molecular biology, November 2016
|
DOI | 10.1007/978-1-4939-6584-7_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6582-3, 978-1-4939-6584-7
|
Authors |
Avijit K. Adak, Ting-Wei Lin, Ben-Yuan Li, Chun-Cheng Lin |
Editors |
Mahesh Uttamchandani, Shao Q. Yao |
Abstract |
The interactions between soluble carbohydrates and/or surface displayed glycans and protein receptors are essential to many biological processes and cellular recognition events. Carbohydrate microarrays provide opportunities for high-throughput quantitative analysis of carbohydrate-protein interactions. Over the past decade, various techniques have been implemented for immobilizing glycans on solid surfaces in a microarray format. Herein, we describe a detailed protocol for fabricating carbohydrate microarrays that capitalizes on the intrinsic reactivity of boronic acid toward carbohydrates to form stable boronate diesters. A large variety of unprotected carbohydrates ranging in structure from simple disaccharides and trisaccharides to considerably more complex human milk and blood group (oligo)saccharides have been covalently immobilized in a single step on glass slides, which were derivatized with high-affinity boronic acid ligands. The immobilized ligands in these microarrays maintain the receptor-binding activities including those of lectins and antibodies according to the structures of their pendant carbohydrates for rapid analysis of a number of carbohydrate-recognition events within 30 h. This method facilitates the direct construction of otherwise difficult to obtain carbohydrate microarrays from underivatized glycans. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Lecturer | 1 | 17% |
Student > Bachelor | 1 | 17% |
Student > Master | 1 | 17% |
Researcher | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Other | 0 | 0% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 50% |
Social Sciences | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 1 | 17% |