| Chapter title |
Annotating Mutational Effects on Proteins and Protein Interactions: Designing Novel and Revisiting Existing Protocols
|
|---|---|
| Chapter number | 17 |
| Book title |
Proteomics
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6747-6_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6745-2, 978-1-4939-6747-6
|
| Authors |
Minghui Li, Alexander Goncearenco, Anna R. Panchenko, Li, Minghui, Goncearenco, Alexander, Panchenko, Anna R. |
| Editors |
Lucio Comai, Jonathan E. Katz, Parag Mallick |
| Abstract |
In this review we describe a protocol to annotate the effects of missense mutations on proteins, their functions, stability, and binding. For this purpose we present a collection of the most comprehensive databases which store different types of sequencing data on missense mutations, we discuss their relationships, possible intersections, and unique features. Next, we suggest an annotation workflow using the state-of-the art methods and highlight their usability, advantages, and limitations for different cases. Finally, we address a particularly difficult problem of deciphering the molecular mechanisms of mutations on proteins and protein complexes to understand the origins and mechanisms of diseases. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 15% |
| Student > Bachelor | 6 | 15% |
| Researcher | 5 | 13% |
| Student > Ph. D. Student | 5 | 13% |
| Other | 3 | 8% |
| Other | 6 | 15% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 28% |
| Agricultural and Biological Sciences | 6 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Computer Science | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 6 | 15% |
| Unknown | 10 | 26% |