| Chapter title |
Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells
|
|---|---|
| Chapter number | 14 |
| Book title |
T follicular Helper Cells
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2498-1_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2497-4, 978-1-4939-2498-1
|
| Authors |
Jan Misiak, Karin Tarte, Patricia Amé-Thomas |
| Editors |
Marion Espéli, Michelle Linterman |
| Abstract |
T-follicular helper (Tfh) cells have emerged as an independent CD4(+) helper T-cell lineage required for antigen-selected germinal center B-cell survival, class switch recombination, and differentiation into long-lived plasma cells. The quantification and function of Tfh subsets are currently extensively explored in humans with infectious diseases, cancer, or autoimmune disorders. Reliable methods to identify and isolate human Tfh cells in patients and healthy donors are necessary to perform these studies. Here, we propose a classical and robust flow cytometric method to detect and isolate Tfh cells from human secondary lymphoid organs based on the expression of CXCR5, PD-1, and CD25 in the CD4(+) T-cell population. An alternative protocol using anti-ICOS and anti-Bcl-6 antibodies and requiring fixation and permeabilization steps without a decrease of detection of membrane markers is also described. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 23% |
| Student > Doctoral Student | 2 | 15% |
| Professor > Associate Professor | 2 | 15% |
| Other | 1 | 8% |
| Professor | 1 | 8% |
| Other | 3 | 23% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 31% |
| Medicine and Dentistry | 3 | 23% |
| Immunology and Microbiology | 2 | 15% |
| Unspecified | 1 | 8% |
| Agricultural and Biological Sciences | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 2 | 15% |