Chapter title |
Analysis of human blood memory T follicular helper subsets.
|
---|---|
Chapter number | 16 |
Book title |
T follicular Helper Cells
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2498-1_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2497-4, 978-1-4939-2498-1
|
Authors |
Bentebibel, Salah-Eddine, Jacquemin, Clement, Schmitt, Nathalie, Ueno, Hideki, Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, Hideki Ueno |
Editors |
Marion Espéli, Michelle Linterman |
Abstract |
Human blood contains a memory counterpart of T follicular helper (TFH) cells. Blood TFH cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory TFH cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory TFH subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory TFH subsets. |
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