Chapter title |
Identification of follicular T helper cells in tissue sections.
|
---|---|
Chapter number | 2 |
Book title |
T follicular Helper Cells
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2498-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2497-4, 978-1-4939-2498-1
|
Authors |
Ding, Yanna, Mountz, John D, Hsu, Hui-Chen, Yanna Ding, John D. Mountz, Hui-Chen Hsu, Mountz, John D. |
Editors |
Marion Espéli, Michelle Linterman |
Abstract |
Follicular T helper (Tfh) cells are a critical population of CD4 T helper cells that are primarily localized in the germinal centers (GCs) to help B cell maturation and antibody production. Tfh cells can be identified in tissue sections based on the expression of a panel of classical Tfh surface makers, transcription marker(s), and effector-function cytokines, as well as by their unique anatomic proximity to other GC cells, including follicular dendritic cells (FDC) and GC B cells. Here, we describe an immunofluorescence staining method for visualization of GC Tfh cells in frozen spleen tissue sections of the autoimmune BXD2 mouse using a confocal imaging strategy. Tfh cells were characterized based on the expression of CD4, CXCR5, Bcl6, IL-21, and IL-17. |
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