| Chapter title |
Retroviral Vector Expression in TCR Transgenic CD4(+) T Cells.
|
|---|---|
| Chapter number | 5 |
| Book title |
T follicular Helper Cells
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2498-1_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2497-4, 978-1-4939-2498-1
|
| Authors |
Choi, Youn Soo, Crotty, Shane, Youn Soo Choi, Shane Crotty |
| Editors |
Marion Espéli, Michelle Linterman |
| Abstract |
The regulation of gene expression is key to understand the function of genes of interest. To explore the biological functions of genes, transgenic knock-in or knockout technologies have served as invaluable tools. While recent advances in molecular biology have introduced new techniques (i.e., CRISPR mediated gene editing) (Cong et al., Science 339(6121):819-823, 2013; Wang et al., Cell 153(4):910-918, 2013) for the generation of transgenic mice in a relatively short period of time, it can still take a long time to test biological hypotheses from scratch to design how to generate knock-in or knockout mice. Here, we describe methods to manipulate gene expression in T cell receptor (TCR) transgenic CD4 T cells, which allow us to investigate gene functions in the study of differentiation pathways of follicular helper T (Tfh) cells. |
X Demographics
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|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
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|---|---|---|
| Unknown | 14 | 100% |
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| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 21% |
| Researcher | 3 | 21% |
| Student > Bachelor | 2 | 14% |
| Student > Doctoral Student | 2 | 14% |
| Student > Postgraduate | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 3 | 21% |
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|---|---|---|
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| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 2 | 14% |