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Zygotic Genome Activation

Overview of attention for book
Cover of 'Zygotic Genome Activation'

Table of Contents

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    Book Overview
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    Chapter 1 Clearance of Maternal RNAs: Not a Mummy’s Embryo Anymore
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    Chapter 2 Link of Zygotic Genome Activation and Cell Cycle Control
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    Chapter 3 Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA During Embryogenesis. - PubMed - NCBI
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    Chapter 4 Gene Expression Analysis in Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
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    Chapter 5 Detection of miRNA in Mammalian Oocytes and Embryos. - PubMed - NCBI
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    Chapter 6 Detection of Bidirectional Promoter-Derived lncRNAs from Small-Scale Samples Using Pre-Amplification-Free Directional RNA-seq Method
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    Chapter 7 Detection and Characterization of Small Noncoding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation
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    Chapter 8 Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
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    Chapter 9 RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos
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    Chapter 10 Detection of RNA Polymerase II in Mouse Embryos During Zygotic Genome Activation Using Immunocytochemistry
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    Chapter 11 Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo
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    Chapter 12 Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos
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    Chapter 13 Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation
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    Chapter 14 Targeted Gene Knockdown in Early Embryos Using siRNA
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    Chapter 15 Generating Mouse Models Using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
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    Chapter 16 CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
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    Chapter 17 Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development
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    Chapter 18 Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes
Attention for Chapter 5: Detection of miRNA in Mammalian Oocytes and Embryos. - PubMed - NCBI
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Chapter title
Detection of miRNA in Mammalian Oocytes and Embryos. - PubMed - NCBI
Chapter number 5
Book title
Zygotic Genome Activation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6988-3_5
Pubmed ID
Book ISBNs
978-1-4939-6986-9, 978-1-4939-6988-3
Authors

Adur, Malavika K, Hale, Benjamin J, Ross, Jason W, Malavika K. Adur, Benjamin J. Hale, Jason W. Ross

Editors

Kiho Lee

Abstract

MicroRNAs (miRNAs) play a crucial role in the regulation of many post-transcriptional processes in reproductive cells. Regulation of maternal mRNA translation and activation of zygotic mRNA are essential to successful embryonic development. Moreover, the precise development of embryonic cell and/or tissue lineages requires temporal and spatial control of gene expression, mRNA abundance, and translation into proteins, which is in part regulated via miRNA. Here, we describe some key protocols that can be utilized to detect and quantify miRNA in in vitro produced oocytes and embryos.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Student > Bachelor 1 25%
Student > Doctoral Student 1 25%
Unknown 1 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%
Medicine and Dentistry 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2017.
All research outputs
#20,418,183
of 22,968,808 outputs
Outputs from Methods in molecular biology
#9,923
of 13,142 outputs
Outputs of similar age
#356,012
of 421,088 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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