| Chapter title |
Generating Mouse Models Using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
|
|---|---|
| Chapter number | 15 |
| Book title |
Zygotic Genome Activation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6988-3_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6986-9, 978-1-4939-6988-3
|
| Authors |
Wenbo Wang, Yingfan Zhang, Haoyi Wang |
| Editors |
Kiho Lee |
| Abstract |
Mouse models with genetic modifications are widely used in biology and biomedical research. Although the application of CRISPR-Cas9 system greatly accelerated the process of generating genetically modified mice, the delivery method depending on manual injection of the components into the embryos remains a bottleneck, as it is laborious, low throughput, and technically demanding. To overcome this limitation, we invented and optimized the ZEN (Zygote electroporation of nucleases) technology to deliver CRISPR-Cas9 reagents via electroporation. Using ZEN, we were able to generate genetically modified mouse models with high efficiency and throughput. Here, we describe the protocol in great detail. |
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