| Chapter title |
CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
|
|---|---|
| Chapter number | 16 |
| Book title |
Zygotic Genome Activation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6988-3_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6986-9, 978-1-4939-6988-3
|
| Authors |
Junghyun Ryu, Kiho Lee |
| Editors |
Kiho Lee |
| Abstract |
Ability to disrupt genes is essential in elucidating gene function. Unlike rodents or amphibians, it has been difficult to generate gene-targeted embryos in large animals. Therefore, studies of early embryo development have been hampered in large animals. A recent technology suggests that targeted mutations can be successfully introduced during embryogenesis, thus by-passing the need of breeding to produce gene-targeted embryos. This is particularly important in large animal models because of longer gestation period and higher animal cost. Here, we describe a specific approach to disrupt up to two genes simultaneously during embryogenesis using the CRISPR/Cas9 technology in swine. The approach can help understand the mechanism of zygotic genome activation in large animals. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 30% |
| Researcher | 2 | 20% |
| Professor | 1 | 10% |
| Other | 1 | 10% |
| Student > Master | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 4 | 40% |
| Social Sciences | 1 | 10% |
| Unknown | 1 | 10% |