| Chapter title |
Detection of RNA Polymerase II in Mouse Embryos During Zygotic Genome Activation Using Immunocytochemistry
|
|---|---|
| Chapter number | 10 |
| Book title |
Zygotic Genome Activation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6988-3_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6986-9, 978-1-4939-6988-3
|
| Authors |
Irina O. Bogolyubova, Dmitry S. Bogolyubov |
| Editors |
Kiho Lee |
| Abstract |
Mammalian pre-implantation embryos represent a highly dynamic experimental model for comparative studies of nuclear structure and functions in the context of gradual reactivation of transcription. Here, we present details of the methods that allow localizing RNA polymerase II in mouse pre-implantation embryos with specific antibodies, using fluorescent/confocal and electron microscopy. We stress the special aspects of immunolabeling protocols in respect to the embryonic material. We made a special emphasis on the essential steps preceding the immunocytochemical experiments. In particular, we consider the procedures of female hormonal stimulation and embryo collection. The described approaches are also applicable to study other nuclear proteins. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 40% |
| Researcher | 1 | 20% |
| Other | 1 | 20% |
| Student > Postgraduate | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 80% |
| Unknown | 1 | 20% |