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Mendeley readers
| Chapter title |
Gene Expression Analysis in Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
|
|---|---|
| Chapter number | 4 |
| Book title |
Zygotic Genome Activation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6988-3_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6986-9, 978-1-4939-6988-3
|
| Authors |
Kyeoung-Hwa Kim, Su-Yeon Lee, Kyung-Ah Lee |
| Editors |
Kiho Lee |
| Abstract |
This chapter describes methods for preparing oocytes and embryos to analyze their gene expression at specific developmental stages. We illustrate how to collect germinal vesicles (GVs) and mature metaphase II (MII) stage oocytes, as well as how to collect embryos at specific developmental stages from the pronucleus (PN) to the blastocyst stage from female mice. We also describe how to prepare mRNAs from these precious cells to analyze the expression of the target genes. The materials and methods in this chapter are used mainly for mouse oocytes and embryos, but with subtle modifications, they may be applicable for most mammalian species. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 50% |
| Student > Doctoral Student | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |