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Mendeley readers
Chapter title |
Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
|
---|---|
Chapter number | 8 |
Book title |
Zygotic Genome Activation
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6988-3_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6986-9, 978-1-4939-6988-3
|
Authors |
Patricia Heyn, Karla M. Neugebauer |
Editors |
Kiho Lee |
Abstract |
Early embryonic development in all known metazoans is characterized by a transcriptionally silent phase, during which development is under control of maternally loaded protein and RNA. The zygotic genome becomes transcriptionally active after a series of rapid reductive cleavage divisions. In this chapter, we present a method to metabolically label, purify, and analyze newly transcribed RNAs in early zebrafish embryos. We previously used this method, which is adaptable to other embryos and systems, to determine the onset of zygotic transcription activation and identify the first zygotic transcripts. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 2 | 33% |
Student > Master | 2 | 33% |
Other | 1 | 17% |
Student > Bachelor | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Unknown | 2 | 33% |