Chapter title |
Expression of Plant Receptor Kinases in Tobacco BY-2 Cells
|
---|---|
Chapter number | 3 |
Book title |
Plant Receptor Kinases
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7063-6_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7062-9, 978-1-4939-7063-6
|
Authors |
Shinohara, Hidefumi, Matsubayashi, Yoshikatsu, Hidefumi Shinohara, Yoshikatsu Matsubayashi |
Abstract |
Although more than 600 single-transmembrane receptor kinase genes have been found in the Arabidopsis genome, only a few of them have known physiological functions, and even fewer plant receptor kinases have known specific ligands. Ligand-binding analysis must be operated using the functionally expressed receptor form. However, the relative abundance of native receptor kinase molecules in the plasma membrane is often quite low. Here, we present a method for stable and functional expression of plant receptor kinases in tobacco BY-2 cells that allows preparation of microsomal fractions containing the receptor. This procedure provides a sufficient amount of receptor proteins while maintaining its ligand-binding activities. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 60% |
Student > Bachelor | 1 | 20% |
Professor > Associate Professor | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 80% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |