Report for: Plant Receptor Kinases

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Chapter title	Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems
Chapter number	11
Book title	Plant Receptor Kinases
Published in	Methods in molecular biology, January 2017
DOI	10.1007/978-1-4939-7063-6_11
Pubmed ID	28567648
Book ISBNs	978-1-4939-7062-9, 978-1-4939-7063-6
Authors	Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, Tatsuya Sawasaki, Nemoto, Keiichirou, Nozawa, Akira, Yamanaka, Satoshi, Nomura, Shunsuke, Kido, Kohki, Sawasaki, Tatsuya
Abstract	The wheat germ cell-free protein synthesis system has a significant advantage for high-throughput production of eukaryotic multidomain proteins in a folded state. In this chapter, we describe two kinds of methods for performing autophosphorylation assay of plant receptor kinases (PRKs) by using the wheat cell-free system. One is an in vitro kinase assay performed using biotin-streptavidin affinity purification technology, and the other is a luminescence-based high-throughput assay for autophosphorylation analysis. We anticipate that our cell-free-based methods might facilitate the characterization of plant PRKs.

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#20,468,008

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#9,951

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#842

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Plant Receptor Kinases

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