| Chapter title |
Purification of Plant Receptor Kinases from Plant Plasma Membranes
|
|---|---|
| Chapter number | 5 |
| Book title |
Plant Receptor Kinases
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7063-6_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7062-9, 978-1-4939-7063-6
|
| Authors |
Lee, Jin Suk, Jin Suk Lee |
| Abstract |
Receptor kinases play a central role in various biological processes, but due to their low abundance and highly hydrophobic and dynamic nature, only a few of them have been functionally characterized, and their partners and ligands remain unidentified. Receptor protein extraction and purification from plant tissues is one of the most challenging steps for the success of various biochemical analyses to characterize their function. Immunoprecipitation is a widely used and selective method for enriching or purifying a specific protein. Here we describe two different optimized protein purification protocols, batch and on-chip immunoprecipitation, which efficiently isolate plant membrane receptor kinases for functional analysis. |
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