Chapter title |
Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases
|
---|---|
Chapter number | 7 |
Book title |
Plant Receptor Kinases
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7063-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7062-9, 978-1-4939-7063-6
|
Authors |
Markus Albert, Ursula Fürst, Albert, Markus, Fürst, Ursula |
Abstract |
The oxidative burst or the production of reactive oxygen species (ROS) is a typical cellular response of both plants and animals to diverse abiotic and biotic stresses. Mainly, the (re-)active oxygen species include the superoxide anion (O2(-)), hydrogen peroxide (H2O2), and the hydroxyl radical (OH•). Here, we outline the detection of extracellularly produced ROS in plant leaf pieces using a chemiluminescence-based bioassay with the luminol L-012 as a substrate being oxidized in the presence of ROS. Since this type of assay is in use in many laboratories, e.g., as a readout for activation of plant receptor kinases, we include a discussion on the interpretation of results and points addressing problems with the buffers at suboptimal pH values that negatively influence the chemiluminescence production. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 5 | 24% |
Student > Ph. D. Student | 3 | 14% |
Other | 1 | 5% |
Student > Doctoral Student | 1 | 5% |
Student > Master | 1 | 5% |
Other | 2 | 10% |
Unknown | 8 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 33% |
Biochemistry, Genetics and Molecular Biology | 5 | 24% |
Environmental Science | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 7 | 33% |