Chapter title |
Protein–Protein Interactions: Co-Immunoprecipitation
|
---|---|
Chapter number | 17 |
Book title |
Bacterial Protein Secretion Systems
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7033-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7031-5, 978-1-4939-7033-9
|
Authors |
Lin, Jer-Sheng, Lai, Erh-Min, Jer-Sheng Lin, Erh-Min Lai |
Editors |
Laure Journet, Eric Cascales |
Abstract |
Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidence shows that protein-protein interactions are crucial in many biological processes in living cells. Genetic (such as yeast two-hybrid, Y2H) and biochemical (such as co-immunoprecipitation, co-IP) methods are the methods commonly used at the beginning of a study to identify the interacting proteins. Immunoprecipitation (IP), a method using a target protein-specific antibody in conjunction with Protein A/G affinity beads, is a powerful tool to identify molecules that interact with specific proteins. Therefore, co-IP is considered to be one of the standard methods of identifying or confirming the occurrence of protein-protein interaction events in vivo. Co-IP experiments can identify proteins via direct or indirect interactions or in a protein complex. Here, we use Agrobacterium type VI secretion system (T6SS) sheath components TssB-TssC41 interaction as an example to describe the principle, procedure, and experimental problems of co-IP. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 318 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 57 | 18% |
Student > Ph. D. Student | 40 | 13% |
Student > Master | 35 | 11% |
Student > Doctoral Student | 15 | 5% |
Researcher | 7 | 2% |
Other | 20 | 6% |
Unknown | 144 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 106 | 33% |
Agricultural and Biological Sciences | 14 | 4% |
Chemistry | 12 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 7 | 2% |
Immunology and Microbiology | 7 | 2% |
Other | 19 | 6% |
Unknown | 153 | 48% |