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Mendeley readers
Chapter title |
Measure of Peptidoglycan Hydrolase Activity
|
---|---|
Chapter number | 12 |
Book title |
Bacterial Protein Secretion Systems
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7033-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7031-5, 978-1-4939-7033-9
|
Authors |
Yoann G. Santin, Eric Cascales |
Editors |
Laure Journet, Eric Cascales |
Abstract |
Most gene clusters encoding multiprotein complexes of the bacterial cell envelope, such as conjugation and secretion systems, Type IV pili, and flagella, bear a gene encoding an enzyme with peptidoglycan hydrolase activity. These enzymes are usually glycoside hydrolases that cleave the glycan chains of the peptidoglycan. Their activities are spatially controlled to avoid cell lysis and to create localized rearrangement of the cell wall. This is assured by interaction with the structural subunits of the apparatus. Here we describe protocols to test the peptidoglycan hydrolase activity of these proteins in vitro and in solution. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 3 | 12% |
Researcher | 3 | 12% |
Student > Doctoral Student | 3 | 12% |
Student > Ph. D. Student | 3 | 12% |
Student > Master | 2 | 8% |
Other | 5 | 20% |
Unknown | 6 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 24% |
Agricultural and Biological Sciences | 5 | 20% |
Immunology and Microbiology | 4 | 16% |
Unspecified | 1 | 4% |
Unknown | 9 | 36% |