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Mendeley readers
| Chapter title |
Defining Membrane Protein Localization by Isopycnic Density Gradients
|
|---|---|
| Chapter number | 6 |
| Book title |
Bacterial Protein Secretion Systems
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7033-9_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7031-5, 978-1-4939-7033-9
|
| Authors |
Rhys A. Dunstan, Iain D. Hay, Trevor Lithgow |
| Editors |
Laure Journet, Eric Cascales |
| Abstract |
In many bacteria, membrane proteins account for around one-third of the proteome and can represent much more than half of the mass of a membrane. Classic techniques in cell biology can be applied to characterise bacterial membranes and their membrane protein constituents. Here we describe a protocol for the purification of outer and inner membranes from Escherichia coli. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Professor | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 43% |
| Immunology and Microbiology | 2 | 29% |
| Chemistry | 1 | 14% |
| Unknown | 1 | 14% |