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Bacterial Protein Secretion Systems

Overview of attention for book
Cover of 'Bacterial Protein Secretion Systems'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder
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    Chapter 2 Protein Sorting Prediction
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    Chapter 3 Cell Fractionation
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    Chapter 4 Defining Lipoprotein Localisation by Fluorescence Microscopy
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    Chapter 5 Identification of Lipoproteins Using Globomycin and Radioactive Palmitate
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    Chapter 6 Defining Membrane Protein Localization by Isopycnic Density Gradients
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    Chapter 7 Cell Surface Exposure
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    Chapter 8 Probing Inner Membrane Protein Topology by Proteolysis
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    Chapter 9 Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM™)
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    Chapter 10 Defining Membrane Protein Topology Using pho-lac Reporter Fusions
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    Chapter 11 In Vivo and In Vitro Protein–Peptidoglycan Interactions
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    Chapter 12 Measure of Peptidoglycan Hydrolase Activity
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    Chapter 13 Protein–Protein Interaction: Bacterial Two-Hybrid
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    Chapter 14 Protein–Protein Interactions: Yeast Two-Hybrid System
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    Chapter 15 Protein–Protein Interactions: Cytology Two-Hybrid
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    Chapter 16 Fusion Reporter Approaches to Monitoring Transmembrane Helix Interactions in Bacterial Membranes
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    Chapter 17 Protein–Protein Interactions: Co-Immunoprecipitation
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    Chapter 18 Protein–Protein Interaction: Tandem Affinity Purification in Bacteria
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    Chapter 19 Site-Directed and Time-Resolved Photocrosslinking in Cells Metabolically Labeled with Radioisotopes
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    Chapter 20 Protein–Protein Interactions: Pull-Down Assays
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    Chapter 21 Protein–Protein Interactions: Surface Plasmon Resonance
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    Chapter 22 Assessing Energy-Dependent Protein Conformational Changes in the TonB System
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    Chapter 23 Defining Assembly Pathways by Fluorescence Microscopy
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    Chapter 24 Large Complexes: Cloning Strategy, Production, and Purification
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    Chapter 25 Shearing and Enrichment of Extracellular Type IV Pili
  27. Altmetric Badge
    Chapter 26 Blue Native PAGE Analysis of Bacterial Secretion Complexes
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    Chapter 27 In Situ Imaging of Bacterial Secretion Systems by Electron Cryotomography
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    Chapter 28 Structural Analysis of Protein Complexes by Cryo Electron Microscopy
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    Chapter 29 Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy
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    Chapter 30 Energy Requirements for Protein Secretion via the Flagellar Type III Secretion System
  32. Altmetric Badge
    Chapter 31 Identification of Effectors: Precipitation of Supernatant Material
  33. Altmetric Badge
    Chapter 32 Screening for Secretion of the Type VI Secretion System Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot
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    Chapter 33 Effector Translocation: Cya Reporter Assay
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    Chapter 34 Monitoring Effector Translocation using the TEM-1 Beta-Lactamase Reporter System
  36. Altmetric Badge
    Chapter 35 Effector Translocation Assay: Differential Solubilization
  37. Altmetric Badge
    Chapter 36 Quantitative Determination of Anti-bacterial Activity During Bacterial Co-culture
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    Chapter 37 Erratum to: Bacterial Filamentous Appendages Investigated by Solid-State NMR Spectroscopy
Attention for Chapter 20: Protein–Protein Interactions: Pull-Down Assays
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

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Citations

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Chapter title
Protein–Protein Interactions: Pull-Down Assays
Chapter number 20
Book title
Bacterial Protein Secretion Systems
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7033-9_20
Pubmed ID
28667618
Book ISBNs
978-1-4939-7031-5, 978-1-4939-7033-9
Authors

Arthur Louche, Suzana P. Salcedo, Sarah Bigot, Louche, Arthur, Salcedo, Suzana P., Bigot, Sarah

Editors

Laure Journet, Eric Cascales

Abstract

Determining protein partners is an essential step toward understanding protein function and identifying relevant biological pathways. Many methods exist for investigating protein-protein interactions. The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein-protein interaction or identifying novel interacting partners. This method typically involves the use of affinity purification with various wash and elution steps. In this chapter, we describe how an interaction between two purified bacterial proteins or between bacterial and eukaryotic proteins can be detected by pull-down experiments.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 349 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 349 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 75 21%
Student > Ph. D. Student 51 15%
Student > Master 42 12%
Student > Doctoral Student 19 5%
Researcher 17 5%
Other 12 3%
Unknown 133 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 132 38%
Agricultural and Biological Sciences 32 9%
Chemistry 11 3%
Immunology and Microbiology 10 3%
Pharmacology, Toxicology and Pharmaceutical Science 8 2%
Other 18 5%
Unknown 138 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2023.
All research outputs
#6,807,800
of 23,967,950 outputs
Outputs from Methods in molecular biology
#2,060
of 13,505 outputs
Outputs of similar age
#104,905
of 316,634 outputs
Outputs of similar age from Methods in molecular biology
#23
of 265 outputs
Altmetric has tracked 23,967,950 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 13,505 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,634 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 265 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.

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