Chapter title |
Phosphoproteomics Analysis for Probing Plant Stress Tolerance
|
---|---|
Chapter number | 11 |
Book title |
Plant Stress Tolerance
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7136-7_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7134-3, 978-1-4939-7136-7
|
Authors |
Christof Rampitsch |
Abstract |
Protein phosphorylation is a key signaling mechanism during the plant biotic and abiotic stress response. Signaling cascades communicate between the cell surface, where the stress is perceived, and the nucleus, where a response can be enacted. Many of these signals involve the specific, transient phosphorylation of proteins by kinases, a signal which is usually amplified through cascades. The advent of high-throughput phosphoproteomics, pioneered mainly in yeast and mammalian cells, has made it possible to discover novel phosphorylation events rapidly and efficiently in a data-dependent manner and this has greatly enlarged our understanding of the plant's response to stress. This chapter describes a simple gel-free protocol for high-throughput phosphoproteomics, which is amenable to most labs engaged in plant stress research. |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 75% |
Unknown | 1 | 25% |