Chapter title |
Detecting Expressed Genes Using CAGE.
|
---|---|
Chapter number | 7 |
Book title |
Transcription Factor Regulatory Networks
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0805-9_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0804-2, 978-1-4939-0805-9
|
Authors |
Mitsuyoshi Murata, Hiromi Nishiyori-Sueki, Miki Kojima-Ishiyama, Piero Carninci, Yoshihide Hayashizaki, Masayoshi Itoh, Murata, Mitsuyoshi, Nishiyori-Sueki, Hiromi, Kojima-Ishiyama, Miki, Carninci, Piero, Hayashizaki, Yoshihide, Itoh, Masayoshi |
Abstract |
Cap analysis of gene expression (CAGE) provides accurate high-throughput measurement of RNA expression. By the large-scale analysis of 5' end of transcripts using CAGE method, it enables not only determination of the transcription start site but also prediction of promoter region. Here we provide a protocol for the construction of no-amplification non-tagging CAGE libraries for Illumina next-generation sequencers (nAnT-iCAGE). We have excluded the commonly used PCR amplification and cleavage of restriction enzyme to eliminate any potential biases. As a result, we achieved less biased simple preparation process. |
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