Chapter title |
Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry
|
---|---|
Chapter number | 18 |
Book title |
Imaging Flow Cytometry
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3302-0_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3300-6, 978-1-4939-3302-0
|
Authors |
Leigh Samsel, J. Philip McCoy, J. Philip McCoyJr. |
Abstract |
Circulating endothelial cells (CECs) are angiogenic cells that appear in increased numbers in the peripheral circulation either as a result of vascular injury or in response to angiogenic stimuli. Elevated levels of CECs have been correlated with various disease states, indicating the use of CECs as a biomarker of disease. Flow cytometry is a widely accepted method for detecting and quantitating CECs. Flow cytometry provides statistical information on large numbers of cells but no information on morphological characteristics. Imaging flow cytometry combines traditional flow cytometry and microscopy, providing a streamlined, multiparameter approach to characterize the biological properties and morphology of large numbers of cells, and is particularly amenable for rare event analysis such as CECs. This approach for identifying and characterizing CECs allows the morphological characterization of large numbers of live, nucleated, single CECs, and alleviates the need for prior enrichment. |
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