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Chapter title |
Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes
|
---|---|
Chapter number | 19 |
Book title |
Imaging Flow Cytometry
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3302-0_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3300-6, 978-1-4939-3302-0
|
Authors |
Lisa M. Niswander, James Palis, Kathleen E. McGrath |
Abstract |
In light of the indispensible role of platelets in the maintenance of hemostasis, understanding the biology of platelet production from bone marrow megakaryocytes (MKs) may uncover new therapeutic strategies for thrombocytopenia. While there has been much recent interest in optimizing culture systems to facilitate the study of the morphologically unique MK lineage, these systems lack the intricacy of in vivo megakaryopoiesis. Given the limitations of many common techniques for the in vivo study of MKs, in this chapter we describe a method to quantify and analyze primary murine bone marrow megakaryocytes utilizing imaging flow cytometry. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 25% |
Professor | 1 | 13% |
Researcher | 1 | 13% |
Student > Bachelor | 1 | 13% |
Student > Postgraduate | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Medicine and Dentistry | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Veterinary Science and Veterinary Medicine | 1 | 13% |
Unknown | 2 | 25% |