Chapter title |
In Vitro Tumorigenic Assay: The Tumor Spheres Assay
|
---|---|
Chapter number | 7 |
Book title |
Cancer Stem Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7401-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7400-9, 978-1-4939-7401-6
|
Authors |
Hui Wang, Anna M. Paczulla, Martina Konantz, Claudia Lengerke |
Abstract |
Cancer stem cells (CSCs) are a subpopulation of cells within cancer tissues that are thought to mediate tumor initiation. CSCs are furthermore considered the cause of tumor progression and recurrence after conventional therapies, based on their enhanced therapy resistance properties. A method commonly used to assess CSC potential in vitro is the so-called tumor spheres assay in which cells are plated under non-adherent culture conditions in serum-free medium supplemented with growth factors. Tumor spheres assays have been used in cancer research as an intermediate in vitro cell culture model to be explored before performing more laborious in vivo tumor xenograft assays. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 40% |
Student > Doctoral Student | 3 | 20% |
Researcher | 2 | 13% |
Student > Bachelor | 1 | 7% |
Student > Master | 1 | 7% |
Other | 1 | 7% |
Unknown | 1 | 7% |
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Social Sciences | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 13% |