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Cancer Stem Cells

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Cover of 'Cancer Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to Cancer Stem Cells: Past, Present, and Future
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    Chapter 2 Surface Markers for the Identification of Cancer Stem Cells
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    Chapter 3 The Role of CD44 and Cancer Stem Cells
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    Chapter 4 Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
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    Chapter 5 Isolation of Cancer Stem Cells by Side Population Method
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    Chapter 6 Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres
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    Chapter 7 In Vitro Tumorigenic Assay: The Tumor Spheres Assay
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    Chapter 8 In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
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    Chapter 9 Xenograft as In Vivo Experimental Model
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    Chapter 10 How to Assess Drug Resistance in Cancer Stem Cells
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    Chapter 11 Tumor Tissue Analogs for the Assessment of Radioresistance in Cancer Stem Cells
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    Chapter 12 Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)
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    Chapter 13 Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System
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    Chapter 14 Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform
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    Chapter 15 Assessing DNA Methylation in Cancer Stem Cells
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    Chapter 16 Histones Acetylation and Cancer Stem Cells (CSCs)
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    Chapter 17 Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods
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    Chapter 18 Circulating Tumor Cells
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    Chapter 19 Erratum to: Introduction to Cancer Stem Cells: Past, Present, and Future
Attention for Chapter 4: Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
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Chapter title
Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
Chapter number 4
Book title
Cancer Stem Cells
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7401-6_4
Pubmed ID
Book ISBNs
978-1-4939-7400-9, 978-1-4939-7401-6
Authors

Luigi Mele, Davide Liccardo, Virginia Tirino

Abstract

The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids. In this chapter, it is described the role of ALDH in the identification of cancer stem cells (CSCs), having been shown that stem cells express high levels of ALDH. Here, we present a method called ALDEFLUOR assay used for the identification, evaluation, and isolation of normal, cancer stem and progenitor cells.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 23%
Student > Bachelor 6 17%
Researcher 5 14%
Student > Master 3 9%
Student > Doctoral Student 2 6%
Other 2 6%
Unknown 9 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 40%
Agricultural and Biological Sciences 2 6%
Chemistry 2 6%
Neuroscience 1 3%
Medicine and Dentistry 1 3%
Other 0 0%
Unknown 15 43%