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Cancer Stem Cells

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Cover of 'Cancer Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to Cancer Stem Cells: Past, Present, and Future
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    Chapter 2 Surface Markers for the Identification of Cancer Stem Cells
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    Chapter 3 The Role of CD44 and Cancer Stem Cells
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    Chapter 4 Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
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    Chapter 5 Isolation of Cancer Stem Cells by Side Population Method
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    Chapter 6 Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres
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    Chapter 7 In Vitro Tumorigenic Assay: The Tumor Spheres Assay
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    Chapter 8 In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
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    Chapter 9 Xenograft as In Vivo Experimental Model
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    Chapter 10 How to Assess Drug Resistance in Cancer Stem Cells
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    Chapter 11 Tumor Tissue Analogs for the Assessment of Radioresistance in Cancer Stem Cells
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    Chapter 12 Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)
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    Chapter 13 Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System
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    Chapter 14 Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform
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    Chapter 15 Assessing DNA Methylation in Cancer Stem Cells
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    Chapter 16 Histones Acetylation and Cancer Stem Cells (CSCs)
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    Chapter 17 Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods
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    Chapter 18 Circulating Tumor Cells
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    Chapter 19 Erratum to: Introduction to Cancer Stem Cells: Past, Present, and Future
Attention for Chapter 17: Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods
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Chapter title
Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods
Chapter number 17
Book title
Cancer Stem Cells
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7401-6_17
Pubmed ID
Book ISBNs
978-1-4939-7400-9, 978-1-4939-7401-6
Authors

Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, Renato Franco

Abstract

Cancer stem cells (CSCs) are rare immortal cells within a tumor that can self-renew and drive tumorigenesis. CSCs play a pivotal role in the tumor development, progression and relapse, as well as in the resistance of anticancer therapy. Different tools could help in the analysis of CSCs, especially Immunohistochemistry (IHC) represents a useful technique able to identify several specific CSC markers. The main aims of this chapter are the description of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 18%
Unspecified 2 12%
Student > Master 2 12%
Researcher 2 12%
Professor 1 6%
Other 3 18%
Unknown 4 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 24%
Unspecified 2 12%
Medicine and Dentistry 2 12%
Neuroscience 2 12%
Nursing and Health Professions 1 6%
Other 2 12%
Unknown 4 24%