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Mendeley readers
| Chapter title |
In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
|
|---|---|
| Chapter number | 8 |
| Book title |
Cancer Stem Cells
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7401-6_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7400-9, 978-1-4939-7401-6
|
| Authors |
Vijayalakshmi Rajendran, Mayur Vilas Jain |
| Abstract |
Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies. |
Mendeley readers
The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 109 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 18% |
| Student > Master | 14 | 13% |
| Student > Bachelor | 13 | 12% |
| Researcher | 9 | 8% |
| Student > Doctoral Student | 9 | 8% |
| Other | 6 | 6% |
| Unknown | 38 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 35 | 32% |
| Agricultural and Biological Sciences | 12 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 6% |
| Medicine and Dentistry | 5 | 5% |
| Business, Management and Accounting | 2 | 2% |
| Other | 9 | 8% |
| Unknown | 40 | 37% |